Phlebotomists must be knowledgeable in both theory and technique. The Phlebotomy Education Program at Saint Luke's Hospital instructs each student in all phases of phlebotomy to include: a basic understanding of anatomy and physiology, the laboratory departments and organization, and in depth knowledge of specimen collection equipment and techniques.

1. Phlebotomy: Past and Present and the Healthcare Setting
2. Quality Assurance and Legal Issues
3. Infection Control, Safety, First Aid, and Personal Wellness
4. Medical Terminology
5. Human Anatomy and Physiology Review
6. The Circulatory System
7. Blood Collection Equipment, Additives, and Order of Draw
8. Venipuncture Procedures
9. Pre-analytical Considerations
10. Capillary Puncture Equipment and Procedures
11. Special Collections and Point-of-Care Testing
12. Arterial Puncture Procedures
13. Non-blood Specimens and Tests
14. Computers and Specimen Handling and Processing
15. Topics in Phlebotomy
16. Practicum